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	Provedor de dados Electron. J. Biotechnol. (2) Autor Alderete,Liliana Marisol (2) Salvio Escandón,Alejandro (2) Hagiwara,Juan Carlos (1) Kato,Adriana (1) Mori,Marcela (1) Palavra-chave Scrophulariaceae (2) Colchicine (1) Flow cytometry (1) In vitro (1) Micropropagation (1) Ornamental plants (1) Tetraploid (1) Mais... Tipo do documento Journal article (2) Ano 2006 (2) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A new variety of Bacopa monnieri obtained by in vitro polyploidization Electron. J. Biotechnol. Salvio Escandón,Alejandro; Hagiwara,Juan Carlos; Alderete,Liliana Marisol. The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences... Tipo: Journal article Palavras-chave: Colchicine; Flow cytometry; Ornamental plants; Scrophulariaceae; Tetraploid. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002 Establishment of an in vitro micropropagation protocol for Mecardonia tenella Electron. J. Biotechnol. Alderete,Liliana Marisol; Mori,Marcela; Kato,Adriana; Salvio Escandón,Alejandro. Mecardonia tenella is an attractive herbaceous native plant, characterized by their intense green foliage and their abundant yellow flowers. It is a very interesting plant for pot and/or garden. A trait to improve in this species is the size of the flowers. The goal of the present paper is to study the in vitro behaviour of M. tenella and to establish a routine protocol for its micropropagation. For the in vitro establishment of M. tenella, nodal segments were disinfected by standard methods using ethanol/sodium hypochloride and cultured on hormone-free MS medium, supplemented with a mixture of antibiotics and an antifungal. In order to study the hormonal requirements of the species, nodal segments were cultured on basal MS supplemented with... Tipo: Journal article Palavras-chave: In vitro; Micropropagation; Scrophulariaceae. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300017 Registros recuperados: 2 Primeira ... 1 ... Última

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